My research involves comparing human and other primate DNA sequences associated with reproduction and stress. We have previously studied promoter sequences regulating the expression of chorionic gonadotropin in humans and the New World primates S. boliviensis and A. trivirgatus. We found that the promoter activity partially explains how chorionic gonadotropin can be expressed in both the pituitary and placenta in New World primates. This is in contrast to humans, where it is expressed only in the placenta. Currently we are studying the promoter sequences of glucocorticoid receptor- associated proteins that are involved in stress and immune function. Our goal is to determine whether the promoter sequences can partially explain the differential expression of these proteins in humans and in New World primates.
Cioffi DL, Hubler TR, Scammell JG. 2011. Organization and function of the FKBP52 and FKBP51 genes. Current Opinions in Pharmacology 11:308-313.
Vasauskas AA, Hubler TR, Boston L, Scammell JG. 2011. Tissue-specific expression of squirrel monkey chorionic gonadotropin. General and Compar. Endocrinology 170:509-513.
Vasauskas AA, Hubler TR, Mahanic C, Gibson S, Kahn AG, Scammell JG. 2011. Regulation and distribution of squirrel monkey chorionic gonadotropin and secretogranin II in the pituitary. General and Comparative Endocrinology 170:514-521.
Gross KL, Westberry JM, Hubler TR, Sadosky PW, Singh RJ, Taylor RL, Scammell JG. 2008. Androgen resistance in squirrel monkeys. Comparative Medicine 58:381-388.
Westberry JM, Sadosky PW, Hubler TR, Gross KL, Scammell JG. 2006. Glucocorticoid resistance in squirrel monkeys results from a combination of a transcriptionally incompetent glucocorticoid receptor and overexpression of the glucocorticoid receptor co-chaperone FKBP51. Journal of Steroid Biochemistry and Molecular Biology 100:34-41.
Scammell JG, Westberry JM, Sadosky PW, Hubler TR, Williams LE, Gibson SV, Singh RJ, Taylor RL, Shackleton CH. 2006. Cortisol metabolism in the Bolivian squirrel monkey (Saimiri boliviensis). Comparative Medicine 56:128-35.
Hubler TR and Scammell JG. 2004. Intronic hormone response elements mediate regulation of FKBP5 by progestins and glucocorticoids. Cell Stress and Chaperones 9: 243-252.
Scammell JG, Hubler TR, Denny WB and Valentine DV. 2003. Organization of the human FK506-binding immunophilin FKBP52 protein gene (FKBP4). Genomics 81:640-643.
Hubler TR, Denny WB, Valentine DV, Cheung-Flynn J, Smith DF and Scammell JG. 2003. The FK506-binding immunophilin FKBP51 is transcriptionally regulated by progestin and attenuates progestin responsiveness. Endocrinology 144:2389-2382.
Hubler, TR and Scammell JG. 2010. Comparative Genomic Analysis of the FKBP51 Promoter in New World and Old World Primates, Saimiri boliviensis, Aotus nancymaae and Homo sapiens. UNA Research Day.
Vasauskas AA, Hubler TR, Scammell JG. 2009. Proximal and distal Egr-1 sites mediate GnRH-responsiveness of the squirrel monkey chrorionic gonadotropin beta-subunit promoter in LbetaT2 cells. FASEB J. 23:598.20.
Hubler TR, Vasauskas AA, Brackin LB, Scammell JG. 2008. Distinct promoter sequences mediate pituitary- and placenta-specific expression of squirrel monkey chorionic gonadotropin beta-subunit. International Primate Genomics Conference: Primate Genomics and Human Disease.
Student research presentations
Caitlin Tidwell. 2012. Does the FKBP5 Promoter Cause Elevated Gene Expression in Squirrel Monkeys? Poster presentation, UNA Research Day.
Chase Mitchell. 2011. Organization of the Chorionic Gonadotropin Beta Subunit Gene in the Owl Monkey, Aotus nancymaae. Poster presentation, Association of Southeastern Biologists, Huntsville AL.
Christina Daily. 2011. Isolation of the Chorionic Gonadotropin Beta Subunit Gene Promoter in the Owl Monkey, Aotus nancymaae. Oral presentation, Association of Southeastern Biologists, Huntsville AL.
Natasha Sanderfer. 2009. Determination of the Promoter Sequence for the Glucocorticord Receptor-Associated Protein FKBP51 in the Squirrel Monkey, Saimiri boliviensis. Poster presentation, UNA Research Day.
Kyle Morton. 2008. Using Comparative Genomics to Isolate the Secretogranin II gene promoter in Saimiri boliviensis. Oral presentation, TriBeta, UNA.
Lori (Brackin) Boston. 2007. Organization of the Chorionic Gonadotropin Beta Subunit Gene in the Squirrel Monkey (Saimiri boliviensis). Oral presentation at NSF-REU Research Day, Oral presentation at TriBeta UNA.
National Center for Biotechnology Information GenBank submissions
JX503530 Saimiri boliviensis FKBP5 gene promoter, 2012.
JN613228 Aotus trivirgatus chorionic gonadotropin promoter and cds, 2011.
GU117708 Saimiri boliviensis chorionic gonadotropin promoter and cds, 2009.
GU132857 Saimiri boliviensis secretogranin II promoter and cds, 2009.